Fig. 5
From: In vitro detection of cancer cells using a novel fluorescent choline derivative
Mean fluorescence intensity (MFI) at increasing incubation time (h) upon incubation with CFA (5 µM). A) MFI of CFA in HeLa cells; B) MFI of CFA in MCF-7 cells. The MFI of samples was normalised by subtracting the background MFI (non-fluorescent area of the negative control) from the MFI of the ROI using Excel. The data are presented as the mean of three independent experiments. The lines shown are error bars. Asterisks indicate statistical significance: *p ≤ 0.05 and ****p ≤ 0.0001; ns, non-significant