Fig. 6
From: In vitro detection of cancer cells using a novel fluorescent choline derivative
Mean fluorescence intensity (MFI). A) MFI in HeLa vs. MCF-7 cells treated with 5 µM of CFA over 0, 2, 4, 6, 16 and 24 h. B) MFI in HeLa or MCF-7 versus other types of cancer cell lines (HepG2 and U-87 MG) and normal cell lines (HEK-293 and HDF) after 24 h incubation with 5 µM of CFA. MFI can be translated to the amount of CFA taken up by cells: the cellular uptake of CFA was time-dependent and significantly lower in other types of tumour cells (HepG2 and U-87 MG) and normal cells. The MFI of samples was normalised by subtracting the background MFI (non-fluorescent area of the negative control) from the MFI of the ROI using Excel. The data are presented as the mean of three independent experiments. The lines shown are error bars. Asterisks indicate statistical significance: **p ≤ 0.01, ***p ≤ 0.001 and ****p ≤ 0.0001; ns, non-significant